Biotechnological analytics

Department

Bachelor's program Biotechnology & Food Engineering

Course unit code

BLT_BA_5_8

Number of ECTS credits allocated

3.0

Name of lecturer(s)

Dr. rer. nat. Losso Klemens

Mode of delivery

face-to-face

Recommended optional program components

none

Recommended or required reading

- Lottspeich, F. et al.: Bioanalytik, Spektrum Akademischer Verlag, 2006.
- Berg, J.M. et al: Biochemie, Spektrum Akademischer Verlag, 2007.
- Voet, D.J. et al.: Lehrbuch der Biochemie, Wiley-VCH, 2002.
- Horton, H.R. et al: Biochemie, Pearson Studium, 2008.

Assessment methods and criteria

Exam

Level of course unit

Bachelor

Year of study

Fall 2025

Semester when the course unit is delivered

5

Language of instruction

English

Learning outcomes of the course unit

Introduction to the processes that are relevant for biotechnology. Familiarization with the methods of cell separation and disintegration, ultrafiltration, aqueous two-phase extraction and chromatography. Ability to describe process stages in qualitative and quantitative terms and to calculate key process parameters. Ability to demonstrate the influence of the main process parameters on efficiency and yield. On completion of the lab course: ability to plan and execute suitable process analytics.

Course contents

Overview of chromatographic methods
• Structure of HPLC: components and working principle
• Theoretical basis: retention factor, resolution, theoretical plates, van Deemter, mass transfer, symmetry, peak widening
• Separation principles: IEC, ALC, SEC, NP, RP, HIC, HILIC, IP-RP
• Stationary phases, functional groups, separation mechanisms, eluent composition
• HPLC optimization, troubleshooting and miniaturization
• Optimization of isocratic and gradient separation, micro- and nano-HPLC
• Other separation methods: CE, SDS-PAGE, ELISA, IEF (fundamentals of separation mechanisms and fields of application)
• MS: ionization techniques (ESI, EI, MALDI, APCI, APPI, FAB), mass analyzers (single/triple quadrupole, 2D/3D ion trap, TOF, ICR, hybrids (qTOF, orbitrap)
• Hyphenated techniques: HPLC-MS (industrial application to biomolecules (proteins, DNA))

Planned learning activities and teaching methods

Teaching methods:
Assessment methods: End of semester exam

Work placement(s)

none