Project Biotechnology 3

Department

Master's Program Biotechnology

Course unit code

BT_MA16_3_11

Number of ECTS credits allocated

4.0

Name of lecturer(s)

Dipl.-Ing. Dr. Hirschl-Neuhauser Sonja, Mag. Ralser Claudia, Bakk. biol., Kaiser Christiane, BSc, Dr. Trockenbacher Alexander

Mode of delivery

face-to-face

Recommended optional program components

none

Recommended or required reading

Sambrook J., Rusell D., Maniatis T.: Molecular cloning: A laboratory manual, 3rd Edition, Cold Spring Harbor Laboratory Press, 2001
Surzycki, S.: Basic Techniques in Molecular Biology, Springer 2000
Mülhardt, C.: Der Experimentator: Molekularbiologie / Genomics, Spektrum Akad. Verlag, 2008
Brown T.A.: Essential molecular biology: a practical approach Vol.1+2 - 2nd Edition, Oxford University Press, 2000
Dyson M, Durocher Y.: Expression systems, Scion Publishing, 2007
McEntyre J., Ostell J., : The NCBI Handbook, National Center for Biotech-nology Information, 2002

Assessment methods and criteria

Course immanent examination

Level of course unit

Master

Year of study

Fall 2025

Semester when the course unit is delivered

3

Language of instruction

English

Learning outcomes of the course unit

Participants in this course are able to plan and perform the induced expression of recombinant proteins in Pichia pastoris in a fermentor. They implement the necessary prearrangements for running the fermentor and are able to perform a batch as well as a fed-batch fermentation.
Graduates of the course are qualified to prepare relevant reports and provide suggestions for optimization.

Course contents

This course is a continuation to the practical course "Project Biotechnology 2". The recombinant Pichia pastoris strains established in the previous project parts will be fermented in a large scale during this course. This includes:
• planning the fermentation
• preparation of media and solutions
• prearrangement of fermenter: calibrations, filling, sterilization
• preparation of preculture and culture
• fermentation: inoculation, sterile sample taking, data logging, induction of protein expression, batch and fed-batch fermentation
• analytics
• evaluation of results:
comparison of growth behavior of native and recombinant strain
comparison of growth behavior in shaking flasks and fermentor
• functional assay for the expressed proteins

Planned learning activities and teaching methods

The practical course comprises an interactive mix of discussions and individual and group work.

Work placement(s)

none