Semester in dem die Lehrveranstaltung angeboten wird
2
Anzahl der zugewiesenen ECTS-Credits
3.0
Name des/der Vortragenden
Dr. Hölzl Georg
Lernergebnisse der Lehrveranstaltung
Derzeit nur in Englisch verfügbar Students receive in-depth knowledge of the analytical methods relevant to biotechnology. Students are particularly familiar with the methods of chromatography and their coupling techniques to spectroscopic methods (mass spectrometry). They are able to describe the process steps qualitatively and quantitatively and to evaluate analytical results and classify them in the production process. The students not only know the corresponding methods for small molecules, but are also familiar with the analytical methods for biomolecules (peptides, proteins, nucleic acids). They can demonstrate the influence of the most important measurement parameters on the selectivity, separation performance and sensitivity of a system. After the internship, the students will be able to plan and perform adequate process analytics.
Art der Veranstaltung
face-to-face
empfohlene optionale Programmeinheiten
Derzeit nur in Englisch verfügbar none
Lehrinhalte
Derzeit nur in Englisch verfügbar Chromatographic techniques, especially HPLC
Basics in Spectroscopy (chromatography related methods)
Mass spectrometry (ionization techniques, instruments and detectors, interpretation of mass spectra)
Fundamentals of NMR
Fundamentals of X-ray crystallography
Protein analytics
Lipid analytics
Glycoprotein analytics
empfohlene Fachliteratur
Derzeit nur in Englisch verfügbar Lottspeich F, Engels JW (eds). Bioanalytics: Analytical methods and concepts in biochemistry and molecular biology. Wiley-VCH Verlag GmbH et Co. KGaA: Weinheim, 2018.
Stryer L, Berg J, Tymoczko J, Gatto G. Biochemistry, 9th edn. Macmillan Learning; WH Freeman: New York, 2019.
Voet D, Voet JG. Biochemistry, 4th edn. John Wiley & Sons: Hoboken, NJ, 2011.
Meyer VR. Practical high-perfomance liquid chromatography, 5th edn. John Wiley: Chichester, 2010.
Gross, J.H.: Mass Spectrometry: A Textbook, Springer, 2011
Lehr- und Lernformen
Derzeit nur in Englisch verfügbar The course comprises an interactive mix of lectures, discussions and individual and group work.